This is often a symptom of camera vignetting, as seen here:

This can usually be resolved by:

  • Adjustments to the optical setup, eg. light source and condenser alignment to be more central, resolving "hot spots".
  • Setup of camera shading correction.

Please contact your distributor for specific instructions on the optical setup of your particular microscope system.

Once you're happy with the optical configuration please follow our guide to setup shading correction in Surveyor:

How to Setup Camera Image (Brightfield)